Device

Part:BBa_K2265000:Design

Designed by: Jenny Nesje   Group: iGEM17_NTNU_Trondheim   (2017-10-17)


MP6 mutator (original)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1413
    Illegal BamHI site found at 2305
    Illegal XhoI site found at 3889
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1285
    Illegal AgeI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4562
    Illegal SapI site found at 961


Design Notes

We had to delete an SpeI cutting site and add flanking suffix and prefix.


Source

The part is a biobrick-friendly version of the MP6 available from AddGene.

References